4707 Highway 61
Suite 184
White Bear Lake, MN 55110-3227 USA
952-449-3946
info@gocleanandclear.com

Wellspring Certificate of Bacteriological Removal Efficiency

The bacteriological filtering capacity of the Clean & Clear Ceramic Wellspring^® Ultra water filter element was independently tested in two ways by Analytical Food Laboratories of Grand Prairie, Texas on June 21, 2006:

	The first test demonstrated the filter's capacity to block 100% of generic E.coli bacteria.
	The second test demonstrated the filter's capacity to block 99.8% of the E.coli O157:H7 bacteria in particular. This strain of E.coli is known to be pathogenic to humans as it produces a powerful toxin, and is a leading cause of food-borne illness, such as the spinach outbreak in September 2006.

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Generic Bacteria Filtration Certification

Ceramic Filter Certificate of Efficiency

This is to certify the Clean & Clear Ceramic Wellspring^® water filter element was tested for bacteriological removal capacity and provided 100% blockage of the bacteria in this trial.

Method

The filter was tested using 5 Liters of inoculated distilled water. The water was inoculated with a live culture of E. coli (generic) of a known concentration. The water was then filtered through the provided apparatus and samples were taken at the beginning, middle and end. Typical growth was not recovered in the filtered water indicating that the filter was able to block passage of the specific bacteria during this trial.

Data & Results

	Initial Concentration of Inoculum: 1.26 x 10⁸.
	Inoculum was diluted to 25,000 cfu/mL and placed in the apparatus.
	From the apparatus aseptic samples were pulled at the beginning of filtering, middle of filtering and end of filtering.
	The samples were then tested using methods from BAM Ch 4.
	Recovery of the bacteria at levels 0.0% or a filtering percentage of 100% indicates that the filter provided blockage of the bacteria in this trial.

Test Certification

Testing was performed on June 21, 2006 by:

Analytical Food Laboratories
865 Greenview Drive
Grand Prairie, Texas 75050-2439
Ph: 800.242.6494 or 972.336.0336
Fax: 972.623.0055
E-mail: afl@afltexas.com
www.afltexas.com

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E.coli O157:H7 Filtration Certification

Ceramic Filter Certificate of Efficiency

This is to certify the Clean & Clear Ceramic Wellspring^® water filter element was tested for bacteriological removal capacity and provided 99.8% blockage of the E.coli O157:H7 bacteria, which is known to be pathogenic to humans.

Method

The filter was tested using 5 Liters of inoculated distilled water. The water was inoculated with a live culture of E. coli O157:H7 of a known concentration. The water was then filtered through the provided apparatus and samples were aseptically taken at the beginning, middle and end. Typical growth was recovered in the filtered water but at lower levels indicating that the filter was able to block passage of the specific bacteria during this trial.

Data & Results

	Initial Concentration of Inoculum: 1.26 x 10⁸
	Inoculum was diluted to 25,000 cfu/mL and placed in the apparatus.
	From the apparatus aseptic samples were pulled at the beginning of filtering, middle of filtering and end of filtering.
	The samples were then tested using methods from BAM Ch 4.
	Recovery of the bacteria at levels of 50 cfu/ml indicates that the filter provided 99.8% blockage of the bacteria. This bacteria was used although it is not normally associated with contaminated water supplies, however it is pathogenic to humans.

Test Certification

Testing was performed on June 21, 2006 by:

Analytical Food Laboratories
865 Greenview Drive
Grand Prairie, Texas 75050-2439
Ph: 800.242.6494 or 972.336.0336
Fax: 972.623.0055
E-mail: afl@afltexas.com
www.afltexas.com

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